Coding

Part:BBa_K2185006:Design

Designed by: Marissa Sumathipala   Group: iGEM16_BroadRun-Baltimore   (2016-09-05)


S.ferax alpha amylase coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 409
    Illegal NgoMIV site found at 745
    Illegal AgeI site found at 620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 936


Design Notes

Codon optimized for S.cerevisiae, kpn and spe sites removed.


Source

It comes from the genome of the fungus S.ferax.

References